Enzyme activity against temperature and pH
Pick an enzyme and change the temperature and pH to see the rate rise to an optimum and fall as the enzyme denatures.
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Questions students ask
What happens when an enzyme is denatured?
The shape of its active site changes, so the substrate no longer fits and the reaction stops. It cannot be reversed.
Why does pepsin work best at pH 2?
It works in the stomach, where hydrochloric acid makes conditions very acidic.
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